antibody anti human cyclin d1  (Cell Signaling Technology Inc)

Journal: Frontiers in Immunology

Article Title: GPER1 signaling restricts macrophage proliferation and accumulation in human hepatocellular carcinoma

doi: 10.3389/fimmu.2024.1481972

Figure Lengend Snippet: GPER1 signaling restrains macrophage proliferation by inhibiting the MEK/ERK/cyclin pathway. (A–D) Human monocyte-derived macrophages were either untreated or treated with 20% Huh7-TSN for 48 hours, in the presence or absence of G-1(1 μM). The levels of p-ERK, ERK, p-Akt, Akt, cyclin D1, cyclin E1, CDK2 and CDK4 were determined using immunoblotting (A, C) . Quantitative analysis of protein expression levels, normalized to β-actin, was performed and plotted ( B, D , n = 4 or 6). The results shown in (B, D) are represented as mean ± SEM. P values were obtained using one-way ANOVA with Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01; ns, not significant.

Article Snippet: Primary Abs used are listed as follows: anti-human Cyclin E1 (4129T, CST), Cyclin D1 (2978T, CST), CDK2 (2546T, CST), CDK4 (12790T, CST), p-AKT (13038S, CST), AKT (4685S, CST), p-Erk1/2 (4370T, CST), Erk1/2 (4695T, CST), β-actin (4970S, CST).

Techniques: Derivative Assay, Western Blot, Expressing

Journal: Discover Oncology

Article Title: PARP1 bound to XRCC2 promotes tumor progression in colorectal cancer

doi: 10.1007/s12672-024-01112-y

Figure Lengend Snippet: Cell proliferation analysis. CCK-8 assay ( A ) and Colony formation assay ( B ) of SW480/SW620 cells transfected with PARP1, PARP1/RNAi, and Vector/Scramble. C Western blot analysis of cyclin D1 and p21 expression. D QPCR analysis of cyclin D1 and p21 expression. Blot membranes were cut prior to hybridisation with antibodies during blotting and developed with X-ray film. *p < 0.05 , (Student’s t test)

Article Snippet: To detect specific proteins, primary antibodies that were used included α-Tubulin mouse monoclonal antibody (1:1000; Sigma-Aldrich, St. Louis, MO, USA), anti-human XRCC2 mouse monoclonal antibody (1:1500; Abcam), anti-human PARP1 mouse monoclonal antibody (1:1500; Abcam), anti-human cyclin D1 rabbit monoclonal antibody (1:1500; Abcam), and anti-human P21 rabbit monoclonal antibody (1:1500; Abcam).

Techniques: CCK-8 Assay, Colony Assay, Transfection, Plasmid Preparation, Western Blot, Expressing, Hybridization

Journal: Journal of Ovarian Research

Article Title: Gene therapy of ovarian cancer using IL-21-secreting human umbilical cord mesenchymal stem cells in nude mice

doi: 10.1186/1757-2215-7-8

Figure Lengend Snippet: The expression of β-catenin and cyclin-D1 in tumor tissues. A . Western blotting results show the expression of β-catenin and cyclin-D1 in the tumor tissues; B . Immunohistochemistry also shows the expression of β-catenin and cyclin-D1. It was found that the expression of β-catenin and cyclin-D1 in tumor tissues from the mice treated with hUCMSCs-LV-IL-21 for four weeks was significantly decreased compared with the hUCMSCs group and hUCMSCs-LV-Vec group or markedly decreased compared with the control group. *p < 0.05, **p < 0.01, and ***p < 0.005. ns: no statistical significant.

Article Snippet: 4 μm formalin-fixed, paraffin embedded slides were incubated with the rabbit anti human β-catenin antibody or rabbit anti human cyclin-D1 antibody (Sigma) after over night incubation at 4°C.

Techniques: Expressing, Western Blot, Immunohistochemistry

Journal: PLoS ONE

Article Title: Wilms’ Tumor Gene 1 (WT1) Silencing Inhibits Proliferation of Malignant Peripheral Nerve Sheath Tumor sNF96.2 Cell Line

doi: 10.1371/journal.pone.0114333

Figure Lengend Snippet: A : Effects of siWT1 on PI3K/Akt/Cyclin D1 pathway in sNF96.2 cells treated with 50 nM p-siWT1 for 48 and 72 hours determined by Western blot analysis. B : Results were reported as fold change compared to siNEG ones (*p<0.05).

Article Snippet: After electrophoresis, proteins were transferred to a nitrocellulose membrane, in a wet system, and proteins transfer was verified by staining membranes with Ponceau S. Membranes were blocked with Tris buffered saline containing 0.01% Tween-20 (TBST) and 5% non-fat dry milk for 1 hour, and then probed overnight at 4°C with the following primary antibodies: rabbit polyclonal anti-WT1 (C-19:sc-192, Santa Cruz Biotechnology Inc, 1∶200), mouse monoclonal anti-PI3K p110 (D-4:sc-8010, Santa Cruz Biotechnology Inc, 1∶200), rabbit polyclonal anti-AKT (9272, Cell Signaling, 1∶1000), mouse monoclonal anti-pAKT (4058, Cell Signaling, 1∶1000), monoclonal rabbit anti-human Cyclin D1 (M3642, Dako, 1∶500), rabbit polyclonal anti-caspase 3, active (8487, Sigma-Aldrich, 1∶1000), rabbit anti-β-actin (A2066, Sigma-Aldrich, 1∶5000), goat polyclonal anti-Lamin A/C (N-18: sc6215, Santa Cruz Biotechnology Inc, 1∶500).

Techniques: Western Blot

Journal: Clinical Medicine Insights. Gastroenterology

Article Title: Eradication of Helicobacter pylori Infection Restores ki67, p53, and Cyclin D1 Immunoreactivity in the Human Gastric Epithelium

doi: 10.4137/CGast.S38330

Figure Lengend Snippet: Histological and immunohistochemical findings at enrollment (T0).

Article Snippet: Immunoexpression of p53, cyclin D1, and ki67 proteins was evaluated with the avidin-biotin complex method using p53 monoclonal mouse anti-human antibody (Clone PA6240; Dako, Glostrup, Denmark), cyclin D1 monoclonal rabbit anti-human antibody (Clone EP12; Dako), and ki67 mouse anti-human antibody (clone MIB-1; Dako).

Techniques: Immunohistochemical staining, Activity Assay, Labeling

Journal: Clinical Medicine Insights. Gastroenterology

Article Title: Eradication of Helicobacter pylori Infection Restores ki67, p53, and Cyclin D1 Immunoreactivity in the Human Gastric Epithelium

doi: 10.4137/CGast.S38330

Figure Lengend Snippet: Histological and immunohistochemical findings in the gastric mucosa of Helicobacter pylori -positive patients at enrollment (T0) and at 6 months after successful eradication (T1).

Article Snippet: Immunoexpression of p53, cyclin D1, and ki67 proteins was evaluated with the avidin-biotin complex method using p53 monoclonal mouse anti-human antibody (Clone PA6240; Dako, Glostrup, Denmark), cyclin D1 monoclonal rabbit anti-human antibody (Clone EP12; Dako), and ki67 mouse anti-human antibody (clone MIB-1; Dako).

Techniques: Immunohistochemical staining, Activity Assay, Labeling

Journal: Clinical Medicine Insights. Gastroenterology

Article Title: Eradication of Helicobacter pylori Infection Restores ki67, p53, and Cyclin D1 Immunoreactivity in the Human Gastric Epithelium

doi: 10.4137/CGast.S38330

Figure Lengend Snippet: Comparison of p53, cyclin D1, and ki67 immunostaining in the gastric mucosa of Helicobacter pylori -negative and H. pylori -eradicated patients.

Article Snippet: Immunoexpression of p53, cyclin D1, and ki67 proteins was evaluated with the avidin-biotin complex method using p53 monoclonal mouse anti-human antibody (Clone PA6240; Dako, Glostrup, Denmark), cyclin D1 monoclonal rabbit anti-human antibody (Clone EP12; Dako), and ki67 mouse anti-human antibody (clone MIB-1; Dako).

Techniques: Immunostaining, Labeling

Journal: Cancers

Article Title: Radioresistance Mechanisms in Prostate Cancer Cell Lines Surviving Ultra-Hypo-Fractionated EBRT: Implications and Possible Clinical Applications.

doi: 10.3390/cancers14225504

Figure Lengend Snippet: Figure 4. Effect of radiation on proliferation and on apoptotic cell markers in PC3RR and DU-145RR cells compared to their parental counterparts. Cell lysates from untreated or cells treated with 4 Gy were collected at 1, 3 and 6 h after IR. Using Western blots, we analysed the expression of Cyclin D1 (A,E), c-FLIPL and c-FLIPS (B,F), BAX (C), Caspase 3 and Cl-Caspase 3 (D,G). β-Actin was used as a control for equal amounts of loaded proteins. Densitometric analysis has been performed on at least three separate Western blots, and the histograms represent the mean ± S.E.M. evaluated as arbitrary units (A.U.). Statistical significance: * p <0.05, ** p <0.01, *** p <0.001; PC3RR vs. PC3 and DU-145RR vs. DU-145 Student’s unpaired t-test.

Article Snippet: Primary antibodies, diluted according to the manufacturer’s instructions, were as follows: mouse anti-human γ-H2AX and total H2AX, mouse anti-human Ku70, mouse anti-human Cancers 2022, 14, 5504 4 of 26 Rad 51, rabbit anti-human Cyclin D1, rabbit anti-human BAX, rabbit anti-human ATF-4 and mouse anti-human OCT-4 from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Western Blot, Expressing, Control